The Furin Polyclonal Antibody (CAB22002) is a valuable tool for researchers studying furin, a proprotein convertase that plays a key role in the processing of several cellular proteins. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.Furin is involved in the cleavage of proproteins into their active forms, making it a crucial player in various physiological processes such as cell growth, differentiation, and migration.
Dysregulation of furin activity has been linked to diseases like cancer, cardiovascular disorders, and infectious diseases.By targeting furin with this polyclonal antibody, researchers can detect and analyze the expression of furin in different cell types, providing valuable insights into its function and potential therapeutic applications. This antibody is a valuable asset for studies in cell biology, biochemistry, and drug development related to furin and its associated pathways.
Product Name:
Furin Polyclonal Antibody
SKU:
CAB22002
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Furin (NP_002560.1).
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. Like other members of this convertase family, the product of this gene specifically cleaves substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140, and may play a role in tumor progression. Unlike SARS-CoV and other coronaviruses, the spike protein of SARS-CoV-2 is thought to be uniquely cleaved by this protease. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
5045
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of HepG2 cells, using Furin antibody (CAB22002) at 1:600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
