The FoxN4 Polyclonal Antibody (PACO62579) is a valuable tool for researchers studying FoxN4, a transcription factor that plays a crucial role in the development of the nervous system. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting.FoxN4 is known to be involved in the differentiation of neurons and the formation of neural circuits, making it a key player in neurodevelopmental processes. Research on FoxN4 is essential for understanding how the nervous system develops and functions, as well as for investigating potential therapeutic targets for neurological disorders.
By using the FoxN4 Polyclonal Antibody, researchers can effectively detect and analyze FoxN4 protein levels in different cell types, providing valuable insights into its physiological functions. This antibody is a valuable tool for studies in neuroscience, developmental biology, and neurodegenerative diseases, offering new opportunities for exploring the intricate mechanisms underlying neural development and function.
Antibody Name:
FOXN4 Antibody (PACO62579)
Antibody SKU:
PACO62579
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Forkhead box protein N4 protein (89-194AA)
Immunofluorescence staining of HepG2 cells with PACO62579 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Transcription factor essential for neural and some non-neural tissues development, such as retina and lung respectively. Binds to an 11-bp consensus sequence containing the invariant tetranucleotide 5'-ACGC-3'. During development of the central nervous system, is required to specify the amacrine and horizontal cell fates from multipotent retinal progenitors while suppressing the alternative photoreceptor cell fates through activating DLL4-NOTCH signaling. Also acts synergistically with ASCL1/MASH1 to activate DLL4-NOTCH signaling and drive commitment of p2 progenitors to the V2b interneuron fates during spinal cord neurogenesis. In development of non-neural tissues, plays an essential role in the specification of the atrioventricular canal and is indirectly required for patterning the distal airway during lung development (By similarity).
Synonyms:
Forkhead box protein N4, FOXN4
UniProt Protein Function:
FOXN4: 2 isoforms of the human protein are produced by alternative splicing.Protein type: Cell development/differentiation; DNA-binding; Transcription factorChromosomal Location of Human Ortholog: 12q24.11Cellular Component: nucleusMolecular Function: chromatin binding; transcription factor activityBiological Process: heart looping; positive regulation of transcription, DNA-dependent; regulation of heart contraction; regulation of transcription, DNA-dependent
UniProt Protein Details:
NCBI Summary:
Members of the winged-helix/forkhead family of transcription factors, such as FOXN4, are characterized by a 110-amino acid DNA-binding domain that can fold into a variant of the helix-turn-helix motif consisting of 3 alpha helices flanked by 2 large loops or wings. These transcription factors are involved in a variety of biologic processes as key regulators in development and metabolism (Li et al., 2004 [PubMed 15363391]).[supplied by OMIM, Mar 2008]
