The FANCD2 Polyclonal Antibody (CAB19311) is a valuable tool for researchers studying the FANCD2 protein, which plays a crucial role in DNA repair and maintenance of genomic stability. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the FANCD2 protein, the antibody enables the detection and analysis of this key player in DNA damage response pathways in a variety of cell types.FANCD2 is a key component of the Fanconi Anemia pathway, a complex network of proteins responsible for repairing DNA damage and preventing the accumulation of mutations.
Dysregulation of this pathway has been linked to a variety of diseases, including cancer and genetic disorders. Understanding the function of FANCD2 is essential for unraveling its role in disease development and potentially developing targeted therapies for conditions associated with DNA repair defects.Overall, the FANCD2 Polyclonal Antibody is a valuable tool for researchers investigating DNA repair mechanisms, genomic stability, and the pathogenesis of diseases linked to defects in DNA repair pathways. Its high specificity and sensitivity make it an ideal choice for studies in cancer biology, genetic disorders, and related fields.
Product Name:
FANCD2 Rabbit Polyclonal Antibody
SKU:
CAB19311
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-291 of FANCD2 (NP_001018125.1).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
FA4; FAD; FACD; FAD2; FA-D2; FANCD; FANCD2
Positive Sample:
K-562,HeLa,A-549,C6,Mouse testis
Cellular Localization:
Nucleus.
Calculated MW:
164kDa
Observed MW:
164kDa
The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
2177
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using FANCD2 Rabbit pAb (CAB19311) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
