The FAM111A Polyclonal Antibody (PAC050914) is a valuable tool for researchers studying FAM111A, a protein involved in DNA replication and repair processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the FAM111A protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential reagent for studies in genetics and cancer research.
FAM111A, also known as DNA helicase Z, plays a crucial role in maintaining genomic stability by facilitating DNA replication and repair mechanisms. Mutations in the FAM111A gene have been linked to genetic disorders and cancer development, highlighting the importance of understanding its function in cellular processes. Research on FAM111A can provide insights into disease mechanisms and potential therapeutic targets for genetic diseases and cancer treatment strategies.
Immunohistochemistry of paraffin-embedded human liver cancer using PACO50914 at dilution of 1:100.
Immunofluorescent analysis of HepG2 cells using PACO50914 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Chromatin-associated protein required for PCNA loading on replication sites. Promotes S-phase entry and DNA synthesis. May directly function at replication forks, explaining why Simian virus 40 (SV40) interacts with FAM111A to overcome host range restriction.
Synonyms:
Protein FAM111A, FAM111A, KIAA1895
UniProt Protein Function:
FAM111A: Belongs to the FAM111 family.Protein type: Unknown functionChromosomal Location of Human Ortholog: 11q12.1Cellular Component: cytoplasm; nucleus; chromatinMolecular Function: protein binding; catalytic activityBiological Process: negative regulation of viral genome replication; viral reproduction; DNA replication; defense response to virusDisease: Kenny-caffey Syndrome, Type 2; Gracile Bone Dysplasia
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is cell-cycle regulated, and has nuclear localization. The C-terminal half of the protein shares homology with trypsin-like peptidases and it contains a PCNA-interacting peptide (PIP) box, that is necessary for its co-localization with proliferating cell nuclear antigen (PCNA). Reduced expression of this gene resulted in DNA replication defects, consistent with the demonstrated role for this gene in Simian Virus 40 (SV40) viral replication. Mutations in this gene have been associated with Kenny-Caffey syndrome (KCS) type 2 and the more severe osteocraniostenosis (OCS, also known as Gracile Bone Dysplasia), both characterized by short stature, hypoparathyroidism, bone development abnormalities, and hypocalcemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]
