The EphB1/EphB2/EphB3/EphB4 Polyclonal Antibody (PAC021598) is a vital tool for research involving the Eph receptors, a group of receptor tyrosine kinases with diverse functions in tissue development, neural connectivity, and tumor progression. This antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it suitable for various research applications, including Western blot analysis.The Eph receptors, including EphB1, EphB2, EphB3, and EphB4, play crucial roles in cell signaling, cell migration, and tissue patterning during development. Dysregulation of these receptors has been implicated in various diseases, including cancer, neurological disorders, and cardiovascular conditions.
By targeting these receptors, researchers can gain insights into the molecular mechanisms underlying these diseases and potentially identify novel therapeutic strategies.The EphB1/EphB2/EphB3/EphB4 Polyclonal Antibody is a valuable tool for studying the expression and activation of these receptors in different cell types and disease states. Its high specificity and sensitivity make it ideal for investigating the roles of Eph receptors in various physiological and pathological processes, paving the way for new discoveries in the field of molecular biology and therapeutics.
Synthesized non-phosphopeptide derived from human EPHB1/2/3/4 around the phosphorylation site of tyrosine 600/602/614/596 (F-T-Y(p)-E-D).
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from 3T3 cells, treated with heat shock, using EPHB1/2/3/4 (Ab-600/602/614/596) antibody.
Background:
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. Beside its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively. Stein E.,Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
EphB1: a receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-B family: ephrin-B1, -B2 and -B3. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. The ligand-activated form of EphB1 interacts with GRB2, GRB10 and NCK through their respective SH2 domains. Four alternatively spliced isoforms are known.
UniProt Protein Details:
Protein type:Protein kinase, TK; Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Membrane protein, integral; TK group; Eph family
Chromosomal Location of Human Ortholog: 3q21-q23
Cellular Component: cytosol; early endosome membrane; extracellular region; integral to plasma membrane; plasma membrane
Biological Process: angiogenesis; axon guidance; cell-substrate adhesion; central nervous system projection neuron axonogenesis; detection of temperature stimulus involved in sensory perception of pain; ephrin receptor signaling pathway; establishment of cell polarity; neurogenesis; positive regulation of synaptogenesis; protein amino acid autophosphorylation; regulation of JNK cascade; retinal ganglion cell axon guidance
NCBI Summary:
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008]
