The ENTR1 Polyclonal Antibody (PACO57956) is a valuable tool for researchers studying the ENTR1 protein, a key player in cellular transport mechanisms. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blotting applications. By targeting the ENTR1 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential component for studies in cell biology and molecular biology.
ENTR1, also known as an endosomal sodium/proton exchanger, plays a critical role in maintaining cellular pH balance and regulating ion transport. Its involvement in these essential processes makes it a promising target for research on cellular homeostasis and function. By understanding the mechanisms by which ENTR1 operates, researchers can gain valuable insights into cellular physiology and potentially uncover new therapeutic strategies for diseases related to ion transport dysfunction.
IHC image of PACO57956 diluted at 1:300 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with PACO57956 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO57956 diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Endosome-associated protein that plays a role in membrane receptor sorting, cytokinesis and ciliogenesis. Involved in the endosome-to-plasma membrane trafficking and recycling of SNX27-retromer-dependent cargo proteins, such as GLUT1. Involved in the regulation of cytokinesis; the function may involve PTPN13 and GIT1. Plays a role in the formation of cilia. Involved in cargo protein localization, such as PKD2, at primary cilia. Involved in the presentation of the tumor necrosis factor (TNF) receptor TNFRSF1A on the cell surface, and hence in the modulation of the TNF-induced apoptosis (By similarity).
Synonyms:
Endosome-associated-trafficking regulator 1 (Antigen NY-CO-3) (Serologically defined colon cancer antigen 3), ENTR1, SDCCAG3
UniProt Protein Function:
SDCCAG3: May be involved in modulation of TNF response. May be involved in presentation of TNFRSF1A on the cell surface. Belongs to the SDCCAG3 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Chromosomal Location of Human Ortholog: 9q34.3
Cellular Component: recycling endosome; retromer complex; early endosome; midbody; endosome
Molecular Function:protein binding
Biological Process: protein transport; cell division; cell cycle; regulation of cytokinesis
