EIF4G2 Rabbit Polyclonal Antibody (CAB2897)
- SKU:
- CAB2897
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
Product Name: | EIF4G2 Rabbit Polyclonal Antibody |
SKU: | CAB2897 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 320-490 of human EIF4G2/p97 (NP_001409.3). |
Sequence: | PKTI NQIR QDAV KDLG VFIP APMA QGMR SDFF LEGP FMPP RMKM DRDP LGGL ADMF GQMP GSGI GTGP GVIQ DRFS PTMG RHRS NQLF NGHG GHIM PPTQ SQFG EMGG KFMK SQGL SQLY HNQS QGLL SQLQ GQSK DMPP RFSK KGQL NADE ISLR PAQS FLMN KNQV PKL |
Tested Applications: | WB IHC-P IF/ICC IP ELISA |
Recommended Dilution: | WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
Synonyms: | P97; AAG1; DAP5; NAT1; EIF4G2/p97 |
Positive Sample: | HeLa,293T,Jurkat,LO2,Mouse brain |
Conjugate: | Unconjugated |
Cellular Localization: | adherens junction, cytosol, eukaryotic translation initiation factor 4F complex |
Calculated MW: | 102kDa |
Observed MW: | 100kDa |
Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
Purification Method: | Affinity purification |
Gene ID: | 1982 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |