The DXO Antibody (PACO49534) is a polyclonal antibody developed for research applications involving DXO, a protein involved in RNA degradation and decay. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot assays. It specifically binds to the DXO protein, allowing for accurate detection and analysis in a variety of cell types.DXO is a key player in RNA metabolism, specifically in the turnover of mRNA molecules. Dysregulation of DXO activity has been implicated in various diseases, including cancer, neurodegenerative disorders, and viral infections.
Understanding the role of DXO in RNA degradation pathways is essential for unraveling its potential as a therapeutic target in these pathological conditions.Overall, the DXO Antibody (PACO49534) serves as a valuable tool for researchers in molecular biology and RNA biology fields, enabling the study of DXO function and its implications in disease development and progression. Its specificity and reliability make it an essential component in advancing our understanding of RNA degradation mechanisms and exploring potential therapeutic interventions.
Immunohistochemistry of paraffin-embedded human testis tissue using PACO49534 at dilution of 1:100.
Immunofluorescent analysis of Hela cells using PACO49534 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Ribonuclease that specifically degrades pre-mRNAs with a defective 5' end cap and is part of a pre-mRNA capping quality control. Has decapping, pyrophosphohydrolase and 5'-3' exonuclease activities. Has decapping activity toward incomplete 5' end cap mRNAs such as unmethylated 5' end-capped RNA to release GpppN and 5' end monophosphate RNA. The 5' end monophosphate RNA is then degraded by the 5'-3' exoribonuclease activity, enabling this enzyme to decap and degrade incompletely capped mRNAs. Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5' end triphosphate to release pyrophosphates (By similarity).
Synonyms:
Decapping and exoribonuclease protein (DXO) (EC 3.1.13) (EC 3.6.1) (Dom-3 homolog Z), DXO, DOM3L DOM3Z NG6
UniProt Protein Function:
DOM3Z: May possess pyrophosphohydrolase activity towards 5' triphosphorylated RNA. Belongs to the Dom3Z family.Protein type: Unknown functionChromosomal Location of Human Ortholog: 6p21.3Cellular Component: cytoplasm; nucleus; plasma membraneMolecular Function: 5'-3' exonuclease activity; magnesium ion binding; mRNA bindingBiological Process: metabolic process; mRNA catabolic process; RNA destabilization
UniProt Protein Details:
NCBI Summary:
This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. The function of its protein product is unknown, but its ubiquitous expression and conservation in both simple and complex eukaryotes suggests that this may be a housekeeping gene. [provided by RefSeq, Jul 2008]
