The DPH7 Polyclonal Antibody (PACO47042) is a valuable tool for researchers studying DPH7, a protein involved in RNA modification and gene expression regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the DPH7 protein, it allows for the detection and analysis of DPH7 in various cell types, making it an essential component for investigations in molecular biology and gene expression studies.DPH7, also known as diphthamide biosynthesis protein 7, plays a crucial role in the biosynthesis of diphthamide, a modified histidine residue found in translation elongation factor 2 (eEF2).
Understanding the function of DPH7 is essential for unraveling the mechanisms of protein synthesis and its dysregulation in various diseases, including cancer and neurological disorders. By targeting DPH7, researchers aim to develop novel therapeutic approaches for addressing these conditions by modulating protein synthesis pathways.
Immunohistochemistry of paraffin-embedded human breast cancer using PACO47042 at dilution of 1:100.
Immunofluorescent analysis of HepG2 cells using PACO47042 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human lung tissue using PACO47042 at dilution of 1:100.
Background:
Catalyzes the demethylation of diphthine methyl ester to form diphthine, an intermediate diphthamide biosynthesis, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2) which can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A (Eta).
Synonyms:
Diphthine methyltransferase (EC 3.1.1.97) (Diphthamide biosynthesis protein 7) (WD repeat-containing protein 85), DPH7, C9orf112 WDR85
UniProt Protein Function:
WDR85: Plays a role in the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in translation elongation factor 2 which can be ADP- ribosylated by diphtheria toxin and by Pseudomonas exotoxin A. Belongs to the WD repeat WDR85 family.Chromosomal Location of Human Ortholog: 9q34.3Biological Process: peptidyl-diphthamide biosynthetic process from peptidyl-histidine
UniProt Protein Details:
NCBI Summary:
WDR85 is a WD repeat-containing protein that plays a role in the first step of diphthamide biosynthesis (Carette et al., 2009 [PubMed 19965467]).[supplied by OMIM, Feb 2010]
