The DOCK4 Polyclonal Antibody (CAB15764) is a valuable tool for researchers studying DOCK4, a protein involved in signaling pathways related to cell migration, adhesion, and cytoskeletal rearrangement. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the DOCK4 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for studies in cell biology and cancer research.
DOCK4, also known as Dedicator of Cytokinesis 4, plays a crucial role in regulating cell migration, which is essential for processes like wound healing and embryonic development. Dysregulation of DOCK4 expression has been linked to cancer metastasis and neurological disorders, making it an important target for further study. Understanding the function of DOCK4 can provide insight into disease mechanisms and may lead to the development of targeted therapies in the future.
Product Name:
DOCK4 Rabbit Polyclonal Antibody
SKU:
CAB15764
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 150-450 of human DOCK4 (NP_055520.3).
This gene is a member of the dedicator of cytokinesis (DOCK) family and encodes a protein with a DHR-1 (CZH-1) domain, a DHR-2 (CZH-2) domain and an SH3 domain. This membrane-associated, cytoplasmic protein functions as a guanine nucleotide exchange factor and is involved in regulation of adherens junctions between cells. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers. Alternatively spliced variants which encode different protein isoforms have been described, but only one has been fully characterized.
Purification Method:
Affinity purification
Gene ID:
9732
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from DU145 cells, using DOCK4 Rabbit pAb (CAB15764) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
