The DNMT3A Polyclonal Antibody (CAB3169) is a valuable tool for researchers studying DNMT3A, a key player in DNA methylation and epigenetic regulation. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it an ideal choice for Western blot applications. By targeting the DNMT3A protein, this antibody enables accurate detection and analysis in various cell types, facilitating research in fields such as cancer biology and developmental biology.DNMT3A, a DNA methyltransferase enzyme, plays a crucial role in establishing and maintaining DNA methylation patterns, which are essential for gene regulation and cellular differentiation.
Dysregulation of DNMT3A has been linked to various diseases, including cancer and developmental disorders, making it a promising target for therapeutic interventions. By studying the function and expression of DNMT3A, researchers can gain insights into the mechanisms underlying these diseases and potentially identify novel treatment strategies.
Product Name:
DNMT3A Rabbit Polyclonal Antibody
SKU:
CAB3169
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 500-700 of human DNMT3A (NP_072046.2).
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
Purification Method:
Affinity purification
Gene ID:
1788
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DNMT3A antibody (CAB3169).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
