The DIRAS1 Polyclonal Antibody (PACO60685) is a vital tool for researchers studying the DIRAS1 protein, a member of the Ras superfamily of small GTPases. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to DIRAS1, this antibody allows for accurate detection and analysis of the protein in a variety of cell types, making it ideal for investigations in cell signaling and cancer research.DIRAS1 is known to play important roles in cellular processes such as cell growth, differentiation, and apoptosis, making it a key target for research into cancer development and progression.
Understanding the function and regulation of DIRAS1 is critical for identifying potential therapeutic targets and developing novel treatments for cancer and other related diseases. The DIRAS1 Polyclonal Antibody serves as a valuable tool for researchers looking to delve deeper into the mechanisms underlying these processes.
IHC image of PACO60685 diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with PACO60685 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Displays low GTPase activity and exists predominantly in the GTP-bound form.
Synonyms:
GTP-binding protein Di-Ras1 (Distinct subgroup of the Ras family member 1) (Ras-related inhibitor of cell growth) (Rig) (Small GTP-binding tumor suppressor 1), DIRAS1, GBTS1 RIG
UniProt Protein Function:
DIRAS1: Displays low GTPase activity and exist predominantly in the GTP-bound form. Belongs to the small GTPase superfamily. Di-Ras family.Protein type: G protein, monomeric, di-Ras; G protein; G protein, monomericChromosomal Location of Human Ortholog: 19p13.3Cellular Component: plasma membraneMolecular Function: GTP binding; GTPase activity; mitogen-activated protein kinase bindingBiological Process: positive regulation of MAP kinase activity
UniProt Protein Details:
NCBI Summary:
DIRAS1 belongs to a distinct branch of the functionally diverse Ras (see HRAS; MIM 190020) superfamily of monomeric GTPases.[supplied by OMIM, Apr 2004]
