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DiMethyl-Histone H4-K20 Polyclonal Antibody (CAB23888)

SKU:
CAB23888
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
$155 - $587
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:DiMethyl-Histone H4-K20 Polyclonal Antibody
SKU:CAB23888
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:A synthetic dimethylated peptide around K20 of human histone H4.
Sequence:HRKV L
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:H4; H4/n; H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4F2; H4FN; FO108; H4-16; H4C11; H4C12; H4C13; H4C15; H4C16; HIST2H4; HIST2H4A; DiMethyl-Histone H4-K20
Positive Sample:HeLa,C6
Conjugate:Unconjugated
Cellular Localization:Chromosome, Nucleus.
Calculated MW:11kDa
Observed MW:11kDa

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Purification Method:Affinity purification
Gene ID:8359
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using DiMethyl-Histone H4-K20 Rabbit pAb (CAB23888) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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