The Derlin-1 Polyclonal Antibody (CAB8508) is a valuable tool for researchers studying Derlin-1, a protein involved in the endoplasmic reticulum-associated protein degradation (ERAD) pathway. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.Derlin-1 is an important component of the ERAD pathway, which helps remove misfolded or unfolded proteins from the endoplasmic reticulum, preventing their accumulation and potential toxicity.
Dysregulation of the ERAD pathway has been implicated in various diseases, including neurodegenerative disorders and cancer, making Derlin-1 a promising target for therapeutic interventions.By using the Derlin-1 Polyclonal Antibody, researchers can accurately detect and analyze Derlin-1 levels in different cell types, providing insights into the role of this protein in health and disease. This antibody is a valuable tool for studies in cell biology, molecular biology, and protein degradation pathways.
Product Name:
DERL1 Rabbit Polyclonal Antibody
SKU:
CAB8508
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 182-251 of human DERL1 (NP_077271.1).
The protein encoded by this gene is a member of the derlin family. Members of this family participate in the ER-associated degradation response and retrotranslocate misfolded or unfolded proteins from the ER lumen to the cytosol for proteasomal degradation. This protein recognizes substrate in the ER and works in a complex to retrotranslocate it across the ER membrane into the cytosol. This protein may select cystic fibrosis transmembrane conductance regulator protein (CFTR) for degradation as well as unfolded proteins in Alzheimer's disease. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
Purification Method:
Affinity purification
Gene ID:
79139
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DERL1 antibody (CAB8508) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
