The DDX28 Polyclonal Antibody (CAB17728) is specifically designed for research involving DDX28, a DEAD-box family protein involved in RNA metabolism and processing. The antibody, raised in rabbits, is highly specific and reactive with human samples, making it suitable for use in Western blot and immunohistochemistry applications. It binds to the DDX28 protein, allowing for detection and analysis in various cellular processes, making it ideal for studies in RNA biology and gene expression regulation.DDX28 is a critical component of cellular RNA pathways, playing a role in RNA splicing, translation, and RNA-protein interactions. Its involvement in RNA processing makes it a key player in gene expression regulation and cellular function.
Understanding the molecular mechanisms of DDX28 can provide insights into how RNA metabolism is regulated and how gene expression is controlled in various physiological and pathological conditions.Overall, the DDX28 Polyclonal Antibody (CAB17728) is a valuable tool for researchers studying RNA biology, gene expression regulation, and cellular processes related to RNA metabolism. With its high specificity and reactivity, it enables accurate detection and analysis of DDX28 in various experimental settings, contributing to advancements in molecular biology and biomedical research.
Product Name:
DDX28 Rabbit Polyclonal Antibody
SKU:
CAB17728
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 371-540 of human DDX28 (NP_060850.2).
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is intronless. It encodes an RNA-dependent ATPase. The encoded protein is localized in the mitochondria and the nucleus, and can be transported between the mitochondria and the nucleus.
Purification Method:
Affinity purification
Gene ID:
55794
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat lung, using DDX28 Rabbit pAb (CAB17728) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
