CYP8B1 Antibody (PACO23506)
- SKU:
- PACO23506
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
CYP8B1 Antibody (PACO23506)
The CYP8B1 Polyclonal Antibody (PAC023506) is a valuable tool for researchers studying the CYP8B1 enzyme, which plays a crucial role in bile acid biosynthesis and cholesterol metabolism. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the CYP8B1 protein, it allows for precise detection and analysis in a variety of cell types, making it an ideal choice for studies in lipid metabolism and liver function.
CYP8B1 is a key enzyme involved in the conversion of cholesterol to bile acids, a process essential for proper digestion and nutrient absorption. Dysregulation of this pathway has been linked to metabolic disorders such as hyperlipidemia and gallstone formation, highlighting the importance of understanding the function of CYP8B1 in health and disease. Research into the role of CYP8B1 may lead to potential therapeutic targets for managing lipid-related disorders and promoting overall metabolic health.
| Antibody Name: | CYP8B1 Antibody (PACO23506) |
| Antibody SKU: | PACO23506 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000, IHC:1:50-1:100 |
| Species Reactivity: | Human |
| Immunogen: | Synthesized peptide derived from internal of human Cytochrome P450 8B1. |
| Form: | Liquid |
| Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts from 293 cells, using Cytochrome P450 8B1 antibody. |
 | Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using Cytochrome P450 8B1 antibody. |
| Background: | Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids. |
| Synonyms: | Cytochrome P450; family 8; subfamily B; polypeptide 1; CYP8B1 |
| UniProt Protein Function: | CYP8B1: Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha- hydroxylated C27 steroids. Belongs to the cytochrome P450 family.Protein type: Lipid Metabolism - primary bile acid biosynthesis; Membrane protein, integral; EC 1.14.13.95; OxidoreductaseChromosomal Location of Human Ortholog: 3p22.1Cellular Component: endoplasmic reticulum membraneMolecular Function: 7alpha-hydroxycholest-4-en-3-one 12alpha-hydroxylase activity; oxygen binding; sterol 12-alpha-hydroxylase activityBiological Process: bile acid biosynthetic process; sterol metabolic process |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UNU6 |
| NCBI GenInfo Identifier: | 308153428 |
| NCBI Gene ID: | 1582 |
| NCBI Accession: | Q9UNU6.2 |
| UniProt Secondary Accession: | Q9UNU6,O75958, Q6NWT2, Q6NWT3, B2RCY3 |
| UniProt Related Accession: | Q9UNU6 |
| Molecular Weight: | 58,068 Da |
| NCBI Full Name: | 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase |
| NCBI Synonym Full Names: | cytochrome P450 family 8 subfamily B member 1 |
| NCBI Official Symbol: | CYP8B1Â Â |
| NCBI Official Synonym Symbols: | CP8B; CYP12Â Â |
| NCBI Protein Information: | 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase |
| UniProt Protein Name: | 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase |
| UniProt Synonym Protein Names: | 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; CYPVIIIB1; Cytochrome P450 8B1; Sterol 12-alpha-hydroxylase |
| Protein Family: | 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase |
| UniProt Gene Name: | CYP8B1Â Â |
| UniProt Entry Name: | CP8B1_HUMAN |
| Antibodies |
| CYP8B1 Antibody (PACO00673) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
Related Products
CYP8B1 Antibody (PACO56776)
€339
CYP8B1 Antibody (PACO00673)
€269
ompF Antibody (PACO64865)
€339
ANKEF1 Antibody (PACO64597)
€339
Ang4 Antibody (PACO64593)
€339
SIM1 Antibody (PACO64949)
€339