The CYP2R1 Polyclonal Antibody (CAB10470) is a valuable tool for researchers studying the cytochrome P450 2R1 enzyme, which plays a crucial role in the conversion of vitamin D to its active form. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.The CYP2R1 enzyme is primarily responsible for converting vitamin D to 25-hydroxyvitamin D in the liver, a critical step in the production of biologically active vitamin D necessary for various physiological processes, including calcium absorption and bone health. Dysregulation of CYP2R1 activity has been linked to vitamin D deficiency, which is associated with a variety of health conditions, including osteoporosis, cardiovascular disease, and cancer.
Researchers can use the CYP2R1 Polyclonal Antibody to detect and analyze the expression of the CYP2R1 enzyme in different cell types and tissues, making it an essential tool for studies in vitamin D metabolism, bone health, and related diseases. By understanding the role of CYP2R1 in vitamin D activation, researchers can develop targeted therapies for conditions associated with vitamin D deficiency and dysregulation of the vitamin D pathway.
Product Name:
CYP2R1 Rabbit Polyclonal Antibody
SKU:
CAB10470
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 252-501 of human CYP2R1 (NP_078790.2).
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor. A mutation in this gene has been associated with selective 25-hydroxyvitamin D deficiency.
Purification Method:
Affinity purification
Gene ID:
120227
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CYP2R1 Rabbit pAb (CAB10470) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
