The CRYGB Polyclonal Antibody (CAB14569) is a versatile tool for researchers studying CRYGB, a protein involved in the formation of the eye lens and known to play a role in cataract development. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the CRYGB protein, this antibody enables accurate detection and analysis in a variety of cell types, making it well-suited for investigations in ophthalmology and vision research.
CRYGB, a key component of the eye lens, is essential for maintaining lens transparency and proper vision. Dysregulation of CRYGB has been linked to various eye disorders, including cataracts, making it a target of interest for studies focused on understanding the molecular mechanisms underlying these conditions. By gaining insights into the function of CRYGB, researchers can contribute to the development of potential treatments and preventive strategies for individuals at risk of developing cataracts or other eye-related issues.
Product Name:
CRYGB Rabbit Polyclonal Antibody
SKU:
CAB14569
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 80-135 of human CRYGB (NP_005201.2).
Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are tandemly organized in a genomic segment as a gene cluster. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation.
Purification Method:
Affinity purification
Gene ID:
1419
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CRYGB Rabbit pAb (CAB14569) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
