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CRYAB Rabbit Monoclonal Antibody (CAB9633)

SKU:
CAB9633
Product Type:
Antibody
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:CRYAB Rabbit Monoclonal Antibody
SKU:CAB9633
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 76-175 of human CRYAB (P02511).
Sequence:SVNL DVKH FSPE ELKV KVLG DVIE VHGK HEER QDEH GFIS REFH RKYR IPAD VDPL TITS SLSS DGVL TVNG PRKQ VSGP ERTI PITR EEKP AVTA APKK
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101; CRYAB
Positive Sample:Rat lung,Rat kidney,Rat heart
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus, Secreted, Lysosome
Calculated MW:20kDa
Observed MW:20kDa

Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants.

Purification Method:Affinity purification
Gene ID:1410
Clone Number:ARC1672
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using CRYAB Rabbit mAb (CAB9633) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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