The CRYAB Rabbit Monoclonal Antibody (CAB9633) is a valuable tool for research involving CRYAB, a protein that plays a key role in maintaining cellular integrity and protecting against stress-induced damage. This antibody, produced in rabbits, is highly specific and sensitive for detecting CRYAB in human samples, making it a reliable choice for Western blot applications. By binding to the CRYAB protein, this antibody enables researchers to study its expression and function in various cell types, providing insights into its role in diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions.
CRYAB, also known as alpha-crystallin B chain or heat shock protein 27, is a molecular chaperone that helps cells cope with stress and maintain proper protein folding. Dysregulation of CRYAB has been implicated in a variety of diseases, making it a promising target for therapeutic interventions. By studying the expression and activity of CRYAB using this antibody, researchers can uncover new insights into its function and potential therapeutic applications in the fields of oncology, neurology, and cardiology.
Product Name:
CRYAB Rabbit Monoclonal Antibody
SKU:
CAB9633
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 76-175 of human CRYAB (P02511).
Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1410
Clone Number:
ARC1672
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using CRYAB Rabbit mAb (CAB9633) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
