Complement factor D Polyclonal Antibody (CAB22110)
The Complement Factor D Polyclonal Antibody is a valuable tool for researchers studying complement factor D, a key component of the alternative complement pathway involved in immune responses and inflammation. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.Complement factor D is essential for the activation of the alternative complement pathway, which plays a crucial role in innate immunity and defense against pathogens. Dysregulation of this pathway has been implicated in various autoimmune diseases, infections, and inflammatory disorders.
By targeting complement factor D, researchers can gain insights into the mechanism of complement activation and its role in immune-related conditions.The Complement Factor D Polyclonal Antibody enables specific detection and analysis of complement factor D in various cell types, making it an ideal tool for research in immunology, inflammation, and infectious diseases. Its ability to recognize complement factor D allows for detailed investigation of its function and regulation, providing valuable information for the development of novel therapies targeting the complement system.
Product Name:
Complement factor D Polyclonal Antibody
SKU:
CAB22110
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant protein of human Complement factor D
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Synonyms:
DF; ADN; PFD; ADIPSIN; Complement factor D
Positive Sample:
THP-1,U-87MG(negative)
Conjugate:
Unconjugated
Cellular Localization:
extracellular exosome, extracellular region
Calculated MW:
27kDa
Observed MW:
27kDa
This gene encodes a member of the S1, or chymotrypsin, family of serine peptidases. This protease catalyzes the cleavage of factor B, the rate-limiting step of the alternative pathway of complement activation. This protein also functions as an adipokine, a cell signaling protein secreted by adipocytes, which regulates insulin secretion in mice. Mutations in this gene underlie complement factor D deficiency, which is associated with recurrent bacterial meningitis infections in human patients. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature protease.
Purification Method:
Affinity purification
Gene ID:
1675
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using ComplementfactorD antibody (CAB22110) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 45s.
