CNOT3 Antibody (PACO55430)
- SKU:
- PACO55430
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | CNOT3 Antibody (PACO55430) |
| Antibody SKU: | PACO55430 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:20-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human CCR4-NOT transcription complex subunit 3 protein (257-395AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO55430 diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO55430 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of emryonic stem (ES) cell identity. |
| Synonyms: | CCR4-NOT transcription complex subunit 3 (CCR4-associated factor 3) (Leukocyte receptor cluster member 2), CNOT3, KIAA0691 LENG2 NOT3 |
| UniProt Protein Function: | NOT3: The CCR4-NOT complex functions as general transcription regulation complex. Belongs to the CNOT2/3/5 family. 3 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 19q13.4Cellular Component: nucleus; cytosol; CCR4-NOT complexMolecular Function: protein bindingBiological Process: regulation of translation; poly(A) tail shortening; transcription, DNA-dependent; regulation of transcription, DNA-dependent; RNA-mediated gene silencing; gene expression; trophectodermal cell differentiation; mRNA catabolic process, deadenylation-dependent decay |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | O75175 |
| NCBI GenInfo Identifier: | 46395626 |
| NCBI Gene ID: | 4849 |
| NCBI Accession: | O75175.1 |
| UniProt Secondary Accession: | O75175,Q9NZN7, Q9UF76 |
| UniProt Related Accession: | O75175 |
| Molecular Weight: | 81,872 Da |
| NCBI Full Name: | CCR4-NOT transcription complex subunit 3 |
| NCBI Synonym Full Names: | CCR4-NOT transcription complex, subunit 3 |
| NCBI Official Symbol: | CNOT3Â Â |
| NCBI Official Synonym Symbols: | NOT3; LENG2; NOT3HÂ Â |
| NCBI Protein Information: | CCR4-NOT transcription complex subunit 3; CCR4-associated factor 3; leukocyte receptor cluster member 2; NOT3 (negative regulator of transcription 3, yeast) homolog |
| UniProt Protein Name: | CCR4-NOT transcription complex subunit 3 |
| UniProt Synonym Protein Names: | CCR4-associated factor 3; Leukocyte receptor cluster member 2 |
| Protein Family: | |
| UniProt Gene Name: | CNOT3Â Â |
| UniProt Entry Name: | CNOT3_HUMAN |
| Antibodies |
| CNOT3 Antibody (PACO08493) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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