The F13A1 Polyclonal Antibody (CAB1461) is a key tool for researchers studying the F13A1 protein, which is involved in blood coagulation and clot formation. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the F13A1 protein, this antibody allows for the detection and analysis of F13A1 in a variety of cell types, making it an essential tool for studies in hemostasis and thrombosis research.F13A1, also known as coagulation factor XIII A chain, plays a crucial role in the final stages of the blood clotting cascade, helping to stabilize blood clots and prevent excessive bleeding.
Dysregulation of F13A1 is associated with various bleeding disorders and thrombotic conditions, highlighting its importance in maintaining proper hemostasis. By studying the function of F13A1, researchers can gain insight into the mechanisms of blood clot formation and develop new strategies for treating clotting disorders.
Product Name:
F13A1 Rabbit Polyclonal Antibody
SKU:
CAB1461
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 600-732 of human F13A1 (NP_000120.2).
This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion.
Purification Method:
Affinity purification
Gene ID:
2162
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using F13A1 Rabbit pAb (CAB1461) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
