The CHRDL2 Polyclonal Antibody (PACO19470) is a valuable tool for researchers studying CHRDL2, a key protein involved in signaling pathways related to bone development and other biological processes. This antibody is produced in rabbits and is highly specific for detecting CHRDL2 in human samples, making it an excellent choice for experiments utilizing Western blot techniques.CHRDL2, also known as chordin-like 2, is known to interact with various signaling molecules and receptors, impacting processes such as bone morphogenesis and cell differentiation. By targeting CHRDL2 with this antibody, researchers can gain insights into the mechanisms underlying these complex biological pathways and potentially discover new therapeutic targets for conditions related to bone health and development.
With its high reactivity and specificity, the CHRDL2 Polyclonal Antibody is a reliable tool for studies in cell biology, developmental biology, and regenerative medicine. By enabling the detection and analysis of CHRDL2 in various experimental settings, this antibody supports advancements in our understanding of the molecular mechanisms governing cellular processes and offers new possibilities for therapeutic interventions.
Antibody Name:
CHRDL2 Antibody (PACO19470)
Antibody SKU:
PACO19470
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:25-1:100
Species Reactivity:
Human
Immunogen:
Synthetic peptide of human CHRDL2
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using PACO19470(CHRDL2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO19470(CHRDL2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
CHRDL2 (chordin-related protein 2), also known as BNF1 (breast tumor novel factor 1) or CHL2 (chordin-like 2), is a 429 amino acid, protein that belongs to the chordin family of proteins. CHRDL2 contains three VWFC (von Willebrand Factor type C) domains and is predominantly expressed in uterus and moderately expressed in prostate, liver, ovary, heart and testis. Due to alternative splicing events, CHRDL2 exists as five isoforms, namely isoform I, isoform II, isoform VII, isoform VIII and isoform IX. Two of these isoforms are secreted, while the other three localize to the cytoplasm. Functioning as a BMP-binding inhibitor, CHRDL2 directly interacts with BMPs and blocks their binding to BMP receptors, thereby inhibiting BMP activity. In breast, lung and colon tumors, CHRDL2 expression is upregulated, suggesting a possible role in tumorigenesis.
Synonyms:
chordin-like 2
UniProt Protein Function:
CHRDL2: May inhibit BMPs activity by blocking their interaction with their receptors. Has a negative regulator effect on the cartilage formation/regeneration from immature mesenchymal cells, by preventing or reducing the rate of matrix accumulation. Implicated in tumor angiogenesis. May play a role during myoblast and osteoblast differentiation, and maturation. 5 isoforms of the human protein are produced by alternative splicing.Protein type: Secreted, signal peptide; SecretedChromosomal Location of Human Ortholog: 11q14Cellular Component: extracellular space; cytoplasmBiological Process: ossification; cartilage development; cell differentiation; negative regulation of BMP signaling pathway
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the chordin family of proteins. Chordin family members are secreted proteins that share a cysteine-rich pro-collagen repeat domain and associate with members of the transforming growth factor beta superfamily. In vitro assays demonstrate a direct interaction between the encoded protein and human activin A. This gene is expressed in many tissues including osteoblasts, where it is differentially expressed during differentiation. In addition, its expression is upregulated in human osteoarthritic joint cartilage, suggesting a role in adult cartilage regeneration. [provided by RefSeq, Jan 2015]
