The CHOP Polyclonal Antibody (PACO22212) is a specialized research tool designed for studies involving the CHOP protein, an important regulator of cellular stress responses and apoptosis. This antibody, produced in rabbits, offers high specificity and sensitivity for detecting CHOP in human samples, making it ideal for applications such as Western blotting.CHOP, also known as C/EBP homologous protein, is a critical mediator of endoplasmic reticulum stress-induced apoptosis and plays a key role in the cellular response to various stress stimuli. Its involvement in cell death pathways and its impact on disease states such as cancer and neurodegenerative disorders make CHOP a compelling target for research.
By utilizing the PACO22212 antibody, researchers can accurately analyze CHOP expression levels in different cell types and tissues, facilitating investigations into the molecular mechanisms underlying stress responses and apoptosis regulation. This antibody is a valuable tool for advancing our understanding of CHOP biology and its potential implications for therapeutic development in a range of pathologies.
Antibody Name:
CHP2 Antibody (PACO22212)
Antibody SKU:
PACO22212
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthesized peptide derived from internal of humanCHP2.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from Jurkat cells, K562 cells and HT-29 cells, using CHP2 antibody.
Background:
Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na+/H+ exchange activity. Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation-induced death. Also plays a role in the regulation of cell proliferation and tumor growth by increasing the phosphatase activity of PPP3CA in a calcium-dependent manner. Activator of the calcineurin/NFAT signaling pathway. Involved in the cytoplasmic translocation of the transcription factor NFATC3 to the nucleus.
Synonyms:
Calcineurin B homologous protein 2; Hepatocellular carcinoma-associated antigen 520; CHP2; HCA520;
UniProt Protein Function:
Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na+/H+ exchange activity. Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation-induced death. Also plays a role in the regulation of cell proliferation and tumor growth by increasing the phosphatase activity of PPP3CA in a calcium-dependent manner. Activator of the calcineurin/NFAT signaling pathway. Involved in the cytoplasmic translocation of the transcription factor NFATC3 to the nucleus.
UniProt Protein Details:
NCBI Summary:
This gene product is a small calcium-binding protein that regulates cell pH by controlling plasma membrane-type Na+/H+ exchange activity. This protein shares sequence similarity with calcineurin B and can bind to and stimulate the protein phosphatase activity of calcineurin A (CnA) and functions in the calcineurin/NFAT (nuclear factor of activated T cells) signaling pathway. Another member of the CHP subfamily, Calcineurin B homologous protein 1, is located on Chromosome 15 and is an inhibitor of calcineurin activity and has a genetic phenotype associated with Parkinson's Disease (OMIM:606988). This gene was initially identified as a tumor-associated antigen and was previously referred to as Hepatocellular carcinoma-associated antigen 520. [provided by RefSeq, Jul 2013]
