The CHML Polyclonal Antibody (PAC063635) is a powerful tool for researchers studying CHML, a protein involved in cell division and growth regulation. This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications. By targeting the CHML protein, this antibody allows for accurate detection and analysis in various cell types, making it ideal for investigations in cell biology and cancer research. CHML, also known as Chp at the end of Myosin II, plays a crucial role in regulating cell cycle progression and promoting cell proliferation.
Its involvement in cellular processes makes it a key player in the development and progression of cancer. By understanding the function of CHML, researchers can gain insights into potential therapeutic targets for cancer treatment and other diseases related to abnormal cell growth. The CHML Polyclonal Antibody provides a valuable tool for unraveling the complexities of CHML biology and its implications in various pathological conditions.
Antibody Name:
CHML Antibody (PACO63635)
Antibody SKU:
PACO63635
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Rab proteins geranylgeranyltransferase component A 2 protein (480-656AA)
Immunofluorescence staining of U251 cells with PACO63635 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than CHM in supporting prenylation of Rab3 family.
Synonyms:
Rab proteins geranylgeranyltransferase component A 2 (Choroideremia-like protein) (Rab escort protein 2) (REP-2), CHML, REP2
UniProt Protein Function:
CHML: Binds unprenylated Rab proteins, presents it to the catalytic Rab GGTase dimer, and remains bound to it after the geranylgeranyl transfer reaction. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than REP-1 in supporting prenylation of Rab3 family. Belongs to the Rab GDI family.Chromosomal Location of Human Ortholog: 1q43Cellular Component: cytoplasm; nucleoplasm; Rab-protein geranylgeranyltransferase complexMolecular Function: Rab GTPase bindingBiological Process: protein geranylgeranylation
UniProt Protein Details:
NCBI Summary:
The product of the CHML gene supports geranylgeranylation of most Rab proteins and may substitute for REP-1 in tissues other than retina. CHML is localized close to the gene for Usher syndrome type II. [provided by RefSeq, Jul 2008]
