The CERS4 Polyclonal Antibody (PAC019926) is a valuable tool for researchers studying CERS4, a key enzyme involved in ceramide synthesis and lipid metabolism. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards CERS4 in human samples, making it perfect for use in Western blot applications. By targeting the CERS4 protein, researchers can accurately detect and analyze its expression in various cell types, offering insights into its role in cellular processes related to lipid metabolism and potentially impacting research in areas such as cancer and metabolic disorders.CERS4, also known as ceramide synthase 4, plays a crucial role in the synthesis of ceramides, which are vital components of cell membranes and are involved in cell signaling pathways.
Dysregulation of ceramide levels has been implicated in various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes. By investigating the function of CERS4, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets for intervention.Overall, the CERS4 Polyclonal Antibody provides a reliable tool for researchers interested in exploring the functions of CERS4 in different biological contexts and may contribute to advancing our understanding of lipid metabolism and its implications for human health.
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using PACO19926(CERS4 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200).
Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-5: Human fetal liver tissue, HT29 cells, HUVEC cells, MCF7 cells, human hepatocellular carcinoma tissue, Primary antibody: PACO19926(CERS4 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds.
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using PACO19926(CERS4 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
The LASS (longevity assurance homolog) family members are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1, LASS2, LASS3, LASS4, LASS5 and LASS6) and they are all involved in sphingolipid synthesis. LASS4 is a 394 amino acid, endoplasmic reticulum, multi-pass membrane protein. LASS4 increases the levels of long ceramides such as C22:0- and C24:0-ceramides. In cells deficient for CLN9, as observed in neuronal ceroid lipofuscinosis (NCL) or Batten disease, LASS4 can increase ceramide levels and partially correct growth and apoptosis.
Synonyms:
ceramide synthase 4
UniProt Protein Function:
Lass4: May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing different fatty acid donors (N-linked stearoyl- (C18) or arachidoyl- (C20) ceramides) in a fumonisin B1-independent manner.Protein type: Membrane protein, multi-pass; Membrane protein, integral; DNA-bindingChromosomal Location of Human Ortholog: 19p13.2Cellular Component: endoplasmic reticulum; endoplasmic reticulum membraneMolecular Function: sphingosine N-acyltransferase activityBiological Process: ceramide biosynthetic process; sphingolipid biosynthetic process
