The CENPU Polyclonal Antibody (PACO16696) is a valuable tool for researchers studying the centromere protein CENPU, which plays a crucial role in chromosome segregation during cell division. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the CENPU protein, this antibody allows for the detection and analysis of CENPU in various cell types, making it ideal for studies in cell biology, genetics, and cancer research.CENPU is essential for the proper functioning of the centromere, a region of the chromosome that is critical for the correct alignment and segregation of chromosomes during cell division.
Dysregulation of CENPU has been implicated in chromosome missegregation, leading to genomic instability and potentially contributing to the development of cancer and other genetic diseases. By understanding the role of CENPU in chromosome segregation, researchers can gain valuable insights into the mechanisms underlying these diseases and develop targeted therapies to treat them.
Antibody Name:
CENPU Antibody (PACO16696)
Antibody SKU:
PACO16696
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:1000-1:2000, IHC:1:25-1:100
Species Reactivity:
Human
Immunogen:
Fusion protein of human CENPU
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using PACO16696(CENPU Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO16696(CENPU Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200).
Background:
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). MLF1IP, or CENPU, is an additional factor required for centromere assembly.
Synonyms:
centromere protein U
UniProt Protein Function:
CENPU: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Cell cycle regulationChromosomal Location of Human Ortholog: 4q35.1Cellular Component: cytosol; microtubule organizing center; nucleoplasm; nucleusMolecular Function: protein bindingBiological Process: DNA replication-independent nucleosome assembly at centromere; sister chromatid cohesion
UniProt Protein Details:
NCBI Summary:
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). MLF1IP, or CENPU, is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM, Mar 2008]
Centromere protein of 50 kDa; CENP-50; Interphase centromere complex protein 24; KSHV latent nuclear antigen-interacting protein 1; MLF1-interacting protein; Polo-box-interacting protein 1
