The CENPP Antibody (PAC016005) is a polyclonal antibody designed for research involving Centromere Protein P (CENPP), a protein that plays a critical role in ensuring proper chromosome segregation during cell division. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various applications such as Western blot and immunofluorescence.CENPP is essential for proper centromere function and chromosome stability, making it a key player in cell division and genome integrity. Dysregulation of CENPP has been implicated in various diseases, including cancer and genetic disorders.
By targeting CENPP with this specific antibody, researchers can study its expression and localization in different cell types, providing valuable insights into its function and potential role in disease development.With its high reactivity and specificity, the CENPP Antibody (PAC016005) is a valuable tool for scientists conducting research in cell biology, genetics, and cancer research. By enabling the detection and analysis of CENPP, this antibody opens up new avenues for understanding the molecular mechanisms underlying chromosome segregation and the implications of CENPP dysregulation in disease.
Antibody Name:
CENPP Antibody (PACO16005)
Antibody SKU:
PACO16005
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:5000, WB:1:500-1:2000
Species Reactivity:
Human, Mouse
Immunogen:
Fusion protein of human CENPP
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: Mouse liver tissue, Primary antibody: PACO16005(CENPP Antibody) at dilution 1/373, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second.
Background:
CENPP is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression. Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex.
Synonyms:
centromere protein P
UniProt Protein Function:
CENPP: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex.Chromosomal Location of Human Ortholog: 9q22.31Cellular Component: nucleoplasm; cytosol; chromosome, pericentric regionBiological Process: nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; mitotic cell cycle
UniProt Protein Details:
NCBI Summary:
CENPP is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).[supplied by OMIM, Mar 2008]
