The CENPP Polyclonal Antibody (PACO16004) is a powerful tool for researchers studying the centromere protein P (CENPP), a key player in chromosome segregation and cell division. This antibody, developed through rabbit immunization, exhibits high reactivity with human samples and has been validated for Western blot applications.CENPP is essential for ensuring accurate chromosome segregation during cell division, making it a critical target for investigation in cell biology and cancer research. By binding specifically to the CENPP protein, this antibody enables precise detection and analysis in various cell types, providing valuable insights into the mechanisms underlying cell division and genomic stability.
The role of CENPP in maintaining genomic integrity and proper cell division makes it a promising target for therapeutic interventions aimed at treating cancer and other diseases characterized by aberrant cell proliferation. Researchers studying cell cycle regulation, mitosis, and chromosomal abnormalities will benefit from the use of this antibody in their experiments, advancing our understanding of key biological processes with implications for disease development and treatment.
Antibody Name:
CENPP Antibody (PACO16004)
Antibody SKU:
PACO16004
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:1000-1:2000, WB:1:200-1:1000
Species Reactivity:
Human
Immunogen:
Fusion protein of human CENPP
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: 293T cells, Primary antibody: PACO16004(CENPP Antibody) at dilution 1/449, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes.
Background:
CENPP is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression. Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex.
Synonyms:
centromere protein P
UniProt Protein Function:
CENPP: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex.Chromosomal Location of Human Ortholog: 9q22.31Cellular Component: nucleoplasm; cytosol; chromosome, pericentric regionBiological Process: nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; mitotic cell cycle
UniProt Protein Details:
NCBI Summary:
CENPP is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).[supplied by OMIM, Mar 2008]
