The CENPO Antibody (PACO16007) is an essential tool for research involving the centromere protein C (CENP-C), a key player in the proper function of the centromere and chromosome segregation during cell division. This polyclonal antibody, raised in rabbits, is highly specific and reactive with human samples, making it suitable for a wide range of applications.CENP-C is essential for the assembly of the kinetochore, a complex protein structure that mediates the attachment of chromosomes to the mitotic spindle during cell division. Dysfunction of CENP-C can lead to chromosome missegregation and aneuploidy, which are hallmark features of cancer cells. Therefore, studying the expression and localization of CENP-C using the PACO16007 antibody can provide valuable insights into the mechanisms underlying cancer development and progression.
In addition to its role in cancer biology, CENP-C has also been implicated in other diseases and cellular processes, such as autoimmune disorders and DNA repair. The PACO16007 antibody can be used in various experimental approaches, including immunofluorescence, immunoprecipitation, and flow cytometry, to investigate the functions of CENP-C in different biological contexts.Overall, the CENPO Antibody (PACO16007) is a versatile and reliable tool for researchers interested in studying the molecular mechanisms of centromere function and chromosome segregation in health and disease. Its high specificity and
Antibody Name:
CENPO Antibody (PACO16007)
Antibody SKU:
PACO16007
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:1000-1:2000, IHC:1:25-1:100
Species Reactivity:
Human
Immunogen:
Fusion protein of human CENPO
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using PACO16007(CENPO Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO16007(CENPO Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200).
Background:
This gene encodes a component of the interphase centromere complex. The encoded protein is localized to the centromere throughout the cell cycle and is required for bipolar spindle assembly, chromosome segregation and checkpoint signaling during mitosis. Alternatively spliced transcript variants encoding multiple protein isoforms have been observed for this gene.
Synonyms:
centromere protein O
UniProt Protein Function:
CENPO: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Modulates the kinetochore-bound levels of NDC80 complex. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Unknown functionChromosomal Location of Human Ortholog: 2p23.3Cellular Component: cytosol; nucleoplasmMolecular Function: protein bindingBiological Process: DNA replication-independent nucleosome assembly at centromere; sister chromatid cohesion
UniProt Protein Details:
NCBI Summary:
This gene encodes a component of the interphase centromere complex. The encoded protein is localized to the centromere throughout the cell cycle and is required for bipolar spindle assembly, chromosome segregation and checkpoint signaling during mitosis. Alternatively spliced transcript variants encoding multiple protein isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]
