The CDC42EP5 Polyclonal Antibody (PACO22247) is a valuable tool for researchers studying CDC42EP5, a protein involved in cell signaling pathways and regulation of cellular functions. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to CDC42EP5, this antibody allows for the detection and analysis of the protein in various cell types, making it an essential component for studies in cell biology, signal transduction, and cancer research.CDC42EP5, also known as CDC42 effector protein 5, is a key player in mediating the functions of the small GTPase CDC42, which regulates actin cytoskeleton dynamics, cell migration, and cell polarity.
Research into the role of CDC42EP5 is important for understanding its impact on cell behavior and its potential implications in disease processes. By investigating the function of CDC42EP5, researchers can gain insights into its involvement in cancer progression, neurodevelopmental disorders, and other pathological conditions. The CDC42EP5 Polyclonal Antibody is a valuable resource for unraveling the complex mechanisms underlying CDC42EP5-mediated cellular processes.
Antibody Name:
CDC42EP5 Antibody (PACO22247)
Antibody SKU:
PACO22247
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse
Immunogen:
Synthesized peptide derived from N-terminal of human BORG3.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from Jurkat cells, using BORG3 antibody.
Background:
Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts. Inhibits MAPK8 independently of CDC42 binding. Controls septin organization and this effect is negatively regulated by CDC42 By similarity.
Synonyms:
binder of Rho GTPases 3; Borg3; Cdc42 effector 5; CEP5;
UniProt Protein Function:
CDC42EP5: Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts. Inhibits MAPK8 independently of CDC42 binding. Controls septin organization and this effect is negatively regulated by CDC42. Belongs to the BORG/CEP family.Chromosomal Location of Human Ortholog: 19q13.42Cellular Component: cytoskeleton; membrane; cytoplasm; plasma membrane; endomembrane systemMolecular Function: GTP-Rho bindingBiological Process: regulation of cell shape; positive regulation of pseudopodium formation; positive regulation of actin filament polymerization; JNK cascade; Rho protein signal transduction
UniProt Protein Details:
NCBI Summary:
CDC42, a small Rho GTPase, regulates the formation of F-actin-containing structures through its interaction with the downstream effector proteins. The protein encoded by this gene is a member of the Borg family of CDC42 effector proteins. Borg family proteins contain a CRIB (Cdc42/Rac interactive-binding) domain. They bind to, and negatively regulate the function of, CDC42. The specific function of this protein has not yet been determined. [provided by RefSeq, Jul 2008]
