The CD40 Polyclonal Antibody (CAB0218) is a valuable tool for researchers studying CD40, a cell surface receptor involved in immune responses and inflammation. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the CD40 protein, this antibody enables precise detection and analysis in various cell types, making it an essential component for studies in immunology and cancer research.CD40 is a crucial player in the immune system, functioning as an activator of immune responses and a mediator of inflammatory processes.
Its role in regulating immune cell activity and promoting immune system function has significant implications for diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions. By unraveling the mechanisms behind CD40's functions, researchers can gain valuable insights for developing targeted therapies to modulate immune responses and combat disease progression.
Product Name:
CD40 Rabbit Polyclonal Antibody
SKU:
CAB0218
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence within amino acids 198-277 of human CD40 (NP_001241.1).
Cell membrane, Secreted, Single-pass type I membrane protein.
Calculated MW:
31kDa
Observed MW:
45kDa
This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Purification Method:
Affinity purification
Gene ID:
958
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Raji cells, using CD40 Rabbit pAb (CAB0218) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
