The Catsper3 Polyclonal Antibody (PAC015941) is a valuable tool for researchers studying Catsper3, a key protein involved in sperm cell function and male fertility. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting and immunofluorescence.Catsper3 is a crucial component of the Catsper ion channel complex, which plays a critical role in sperm cell hyperactivation and fertilization. Dysregulation of Catsper3 has been implicated in male infertility, making it a target of interest in reproductive biology research.
By targeting Catsper3 with this antibody, researchers can effectively study the expression and localization of the protein in different cell types, providing insights into its function in sperm physiology.Overall, the Catsper3 Polyclonal Antibody is a valuable tool for investigating the role of Catsper3 in sperm function and male fertility, with potential applications in reproductive medicine and assisted reproduction techniques. Its high specificity and reactivity make it a reliable choice for researchers looking to advance their understanding of sperm cell biology.
Antibody Name:
CATSPER3 Antibody (PACO15941)
Antibody SKU:
PACO15941
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:5000, WB:1:500-1:2000
Species Reactivity:
Human, Mouse
Immunogen:
Fusion protein of human CATSPER3
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Mouse testis tissue, Primary antibody: PACO15941(CATSPER3 Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes.
Background:
CatSpers (cation channel, sperm associated proteins) are ion transport proteins located on the surface of sperm cells in the principal piece of the sperm tail. CatSpers are vital to sperm motility, fertilization and cAMP-mediated calcium influx in sperm. There are four CatSper proteins in mammalian sperm, namely CatSper (or CatSper1), CatSper2, CatSper3 and CatSper4. CatSper proteins contain a single, six-transmembrane-spanning segment and exhibit the voltage-dependent Ca2+ channel four-repeat structure. CatSper proteins are believed to assemble into a heterotetrameric complex, forming an alkalinization-activated Ca2+-selective channel. Mutations in any of the genes encoding CatSper family proteins can result in male infertility. CatSper3 plays an important role in the hyperactivated motility of sperm cells, a process that is required in the preparation of sperm for fertilization.
