The Casp5 Polyclonal Antibody (CAB16733) is a valuable tool for researchers studying the role of Casp5, a caspase enzyme involved in various cellular processes, including apoptosis and inflammation. This antibody, produced in rabbits, is highly specific to human Casp5 and has been validated for use in Western blot applications. Its ability to bind to the Casp5 protein enables precise detection and analysis in a variety of cell types, making it ideal for investigations in the fields of immunology, cancer research, and inflammatory diseases.Casp5, also known as caspase-5, plays a crucial role in the regulation of cell death and inflammation by cleaving specific proteins in response to cellular stress and immune signals.
Dysregulation of Casp5 has been linked to various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions. By understanding the function and activity of Casp5, researchers can gain insights into potential therapeutic targets for these diseases and develop novel treatment strategies that target Casp5-mediated pathways.Overall, the Casp5 Polyclonal Antibody (CAB16733) is a valuable tool for researchers seeking to explore the diverse functions of Casp5 in health and disease. Its high specificity and sensitivity make it a reliable choice for studying the mechanisms underlying Casp5-mediated processes and developing targeted therapies for related disorders.
Product Name:
Caspase-5 Polyclonal Antibody
SKU:
CAB16733
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant Protein corresponding to a sequence within amino acids 140-250 of human Caspase-5 (NP_001129584).
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene.
Purification Method:
Affinity purification
Gene ID:
838
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from THP-1 cells using Caspase-5 Rabbit pAb (CAB16733) at 1:700 dilution. THP-1 cells were treated by TPA (80nM) overnight and LPS (1 μg/ml) at 37 ℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
