The CASC3 Monoclonal Antibody (PAC015935) is a powerful tool for researchers studying CASC3, a key protein involved in RNA metabolism and splicing. This antibody, produced using hybridoma technology, offers high specificity and sensitivity for detecting CASC3 in human samples, making it an essential component for experiments such as Western blotting and immunofluorescence. By binding specifically to CASC3, this antibody allows for precise visualization and analysis of this important protein in a variety of cell types.CASC3 is a critical player in the regulation of gene expression and mRNA processing, making it a target of interest in various fields of research, including cancer biology and developmental biology.
Its role in RNA splicing and stability makes it essential for proper cellular function, and understanding its mechanisms can provide valuable insights into disease pathways and potential therapeutic interventions. By using the CASC3 Monoclonal Antibody, researchers can further investigate the functions and interactions of CASC3, advancing our knowledge of molecular processes underlying normal and disease states.
Antibody Name:
CASC3 Antibody (PACO15935)
Antibody SKU:
PACO15935
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:1000-1:2000, IHC:1:25-1:100
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Fusion protein of human CASC3
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using PACO15935(CASC3 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200).
Background:
The product of this gene is a core component of the exon junction complex (EJC), a protein complex that is deposited on spliced mRNAs at exon-exon junctions and functions in nonsense-mediated mRNA decay (NMD). The encoded protein binds RNA and interacts with two other EJC core components. It is predominantly located in the cytoplasm, but shuttles into the nucleus where it localizes to nuclear speckles.
Synonyms:
cancer susceptibility candidate 3
UniProt Protein Function:
CASC3: Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Stimulates the ATPase and RNA- helicase activities of EIF4A3. Plays a role in the stress response by participating in cytoplasmic stress granules assembly and by favoring cell recovery following stress. Component of the dendritic ribonucleoprotein particles (RNPs) in hippocampal neurons. May play a role in mRNA transport. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Binds poly(G) and poly(U) RNA homopolymer. Belongs to the CASC3 family.
UniProt Protein Details:
Protein type:RNA-binding; Translation
Chromosomal Location of Human Ortholog: 17q21.1
Cellular Component: perinuclear region of cytoplasm; nuclear speck; cytosol; ribonucleoprotein complex
Molecular Function:identical protein binding; protein binding; enzyme binding; ubiquitin protein ligase binding
The product of this gene is a core component of the exon junction complex (EJC), a protein complex that is deposited on spliced mRNAs at exon-exon junctions and functions in nonsense-mediated mRNA decay (NMD). The encoded protein binds RNA and interacts with two other EJC core components. It is predominantly located in the cytoplasm, but shuttles into the nucleus where it localizes to nuclear speckles. [provided by RefSeq, Jul 2008]
protein CASC3; MLN 51; barentsz; protein barentsz; metastatic lymph node 51; metastatic lymph node gene 51 protein; cancer susceptibility candidate gene 3 protein
UniProt Protein Name:
Protein CASC3
UniProt Synonym Protein Names:
Cancer susceptibility candidate gene 3 protein; Metastatic lymph node gene 51 protein; MLN 51; Protein barentsz; Btz
