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Canine Creatine Kinase, Muscle (CKM) ELISA Kit

SKU:
CNDL00019
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P05123
Sensitivity:
3.1U/L
Range:
6.25-400U/L
ELISA Type:
Sandwich
Synonyms:
CK-MM,CKMM,CK3,CK-M,MM-CK,M-CK,Creatine kinase M-type, N-terminally processed
Reactivity:
Canine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Canine Creatine Kinase, Muscle (CKM) ELISA Kit

The Canine Creatine Kinase Muscle (CKM) ELISA Kit is a cutting-edge tool designed for the accurate measurement of CKM levels in canine serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it an invaluable asset for a variety of research applications.Creatine Kinase Muscle (CKM) is a key enzyme found in muscle tissue, playing a vital role in energy metabolism. Elevated levels of CKM are often associated with muscle damage, making it a valuable biomarker for monitoring muscle health and assessing the impact of exercise and physical activity.

Whether studying muscle physiology, evaluating muscle-related disorders, or monitoring the effects of therapeutic interventions, the Canine CKM ELISA Kit provides researchers with a powerful tool for advancing their understanding of canine muscle biology and improving the well-being of canine patients.

Product Name:Canine Creatine Kinase, Muscle (CKM) ELISA Kit
Product Code:CNDL00019
Size:96 Assays
Target:CKM
Synonyms:CK-MM, CKMM, CK3, CK-M, MM-CK, M-CK, Creatine kinase M-type, N-terminally processed
Detection Method:Sandwich
Range:6.25-400 U/L
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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