The CABP4 Polyclonal Antibody (PACO57684) is a vital tool for researchers studying CABP4, a calcium-binding protein involved in neuronal signaling and synaptic transmission. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting CABP4 in human samples, making it ideal for use in various research applications such as Western blotting.CABP4 is essential for proper functioning of the visual system, with mutations in the gene encoding this protein linked to disorders like congenital stationary night blindness.
By targeting and detecting CABP4, researchers can gain insights into the mechanisms underlying visual processing and potentially develop new treatments for related conditions.With its ability to accurately detect CABP4 in different cell types and tissues, the PACO57684 antibody is a valuable tool for advancing our understanding of neuronal function and neurological disorders. Its reliability and specificity make it a valuable asset for studies focused on synaptic transmission, vision, and related physiological processes.
IHC image of PACO57684 diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with PACO57684 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Involved in normal synaptic function through regulation of Ca(2+) influx and neurotransmitter release in photoreceptor synaptic terminals and in auditory transmission. Modulator of CACNA1D and CACNA1F, suppressing the calcium-dependent inactivation and shifting the activation range to more hyperpolarized voltages (By similarity).
Synonyms:
Calcium-binding protein 4 (CaBP4), CABP4
UniProt Protein Function:
CABP4: Involved in normal synaptic function through regulation of Ca(2+) influx and neurotransmitter release in photoreceptor synaptic terminals and in auditory transmission. Modulator of CACNA1D and CACNA1F, suppressing the calcium-dependent inactivation and shifting the activation range to more hyperpolarized voltages. Defects in CABP4 are the cause of congenital stationary night blindness type 2B (CSNB2B). Congenital stationary night blindness is a non-progressive retinal disorder characterized by impaired night vision. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Calcium-bindingChromosomal Location of Human Ortholog: 11q13.2Cellular Component: extracellular region; terminal button; synapse; cytosolMolecular Function: calcium ion bindingBiological Process: visual perception; retinal cone cell development; phototransduction; photoreceptor cell morphogenesis; signal transduction; retinal bipolar neuron differentiationDisease: Night Blindness, Congenital Stationary, Type 2b
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the CABP family of calcium binding protein characterized by four EF-hand motifs. Mutations in this gene are associated with congenital stationary night blindness type 2B. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
