The BOLA3 Polyclonal Antibody (CAB15985) is a valuable tool for researchers studying BOLA3, a protein involved in iron-sulfur cluster biogenesis. This antibody is raised in rabbits and has been validated for use in Western blot applications with human samples. By specifically binding to the BOLA3 protein, this antibody enables accurate detection and analysis in a variety of cell types.BOLA3 plays a crucial role in cellular iron homeostasis and is essential for the proper functioning of iron-sulfur cluster-containing proteins.
Research into the function of BOLA3 is important for understanding the mechanisms underlying various diseases, including metabolic disorders and neurodegenerative diseases. By using the BOLA3 Polyclonal Antibody, researchers can delve into the biological processes involving BOLA3 and potentially uncover new therapeutic targets for these conditions.
Product Name:
BOLA3 Rabbit Polyclonal Antibody
SKU:
CAB15985
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-68 of human BOLA3 (NP_997717.2).
This gene encodes a protein that plays an essential role in the production of iron-sulfur (Fe-S) clusters for the normal maturation of lipoate-containing 2-oxoacid dehydrogenases, and for the assembly of the mitochondrial respiratory chain complexes. Mutation in this gene has been associated with multiple mitochondrial dysfunctions syndrome-2. Two alternatively spliced transcript variants encoding different isoforms with distinct subcellular localization have been reported for this gene (PMID:21944046).
Purification Method:
Affinity purification
Gene ID:
388962
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using BOLA3 Rabbit pAb (CAB15985) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
