The BCAT1 Polyclonal Antibody (CAB16351) is a high-quality antibody designed for research involving BCAT1, a cytosolic enzyme involved in the catabolism of branched-chain amino acids. This antibody, generated in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the BCAT1 protein, this antibody allows for the detection and analysis of BCAT1 expression in a variety of cell types, making it an excellent tool for studies in metabolism and cancer research.BCAT1 plays a crucial role in regulating cellular metabolism by catalyzing the first step in the breakdown of branched-chain amino acids, which are essential for protein synthesis and energy production. Dysregulation of BCAT1 expression has been linked to various diseases, including cancer, metabolic disorders, and neurological conditions.
Therefore, studying the function of BCAT1 is vital for understanding the mechanisms underlying these pathologies and developing targeted therapies to treat them effectively.In conclusion, the BCAT1 Polyclonal Antibody (CAB16351) offers researchers a reliable tool for investigating the role of BCAT1 in cellular metabolism and disease pathogenesis. With its high specificity and sensitivity, this antibody is well-suited for a wide range of experimental applications, providing valuable insights into the molecular mechanisms governing branched-chain amino acid metabolism and its implications for human health.
Product Name:
BCAT1 Rabbit Polyclonal Antibody
SKU:
CAB16351
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-190 of human BCAT1 (NP_005495.2).
This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described.
Purification Method:
Affinity purification
Gene ID:
586
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using BCAT1 Rabbit pAb (CAB16351) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
