The ATRX Polyclonal Antibody (CAB17378) is a valuable tool for researchers studying the ATRX protein, a key player in chromatin remodeling and gene expression regulation. This antibody, raised in rabbits, has high reactivity with human, mouse, and rat samples, and has been validated for use in various applications such as Western blot and immunohistochemistry.ATRX is known for its role in maintaining genome stability and telomere length, making it an important factor in cell proliferation and differentiation. Dysregulation of ATRX has been associated with various cancers, developmental disorders, and neurodegenerative diseases, highlighting its potential as a therapeutic target.
By using the ATRX Polyclonal Antibody, researchers can accurately detect and analyze the expression levels of ATRX in different cell types and tissues, providing valuable insights into its function in normal physiological processes and disease states. This antibody is a valuable tool for studies in molecular biology, cancer research, and developmental biology, allowing for a deeper understanding of ATRX-mediated pathways and potential therapeutic interventions.
Product Name:
ATRX Polyclonal Antibody
SKU:
CAB17378
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 2100-2200 of human ATRX (NP_000480.3).
nuclear body, nuclear chromosome, nucleoplasm, nucleus, PML body
Calculated MW:
283kDa
Observed MW:
310kDa
The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Mutations in this gene are associated with X-linked syndromes exhibiting cognitive disabilities as well as alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.
Purification Method:
Affinity purification
Gene ID:
546
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using ATRX Rabbit pAb (CAB17378) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Negative control (NC): U-2 OS.Exposure time: 60s.
