The ATP6V1E1 Polyclonal Antibody (CAB3756) is a valuable tool for researchers studying ATP6V1E1, a key component of the vacuolar ATPase (V-ATPase) complex. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting ATP6V1E1 in human samples, making it suitable for use in Western blotting applications. By targeting the ATP6V1E1 protein, this antibody enables precise detection and analysis in various cell types, making it an essential asset for studies in cellular biology and metabolic research.ATP6V1E1 is an essential subunit of the V-ATPase complex, playing a crucial role in regulating intracellular pH and vesicular trafficking.
Dysregulation of ATP6V1E1 has been implicated in various diseases, including cancer, osteopetrosis, and lysosomal storage disorders. Understanding the function and localization of ATP6V1E1 is essential for deciphering its role in disease pathology and developing targeted therapies.In conclusion, the ATP6V1E1 Polyclonal Antibody (CAB3756) is a reliable tool for researchers aiming to investigate the role of ATP6V1E1 in cellular processes and disease development. Its high specificity and sensitivity make it a valuable resource for advancing knowledge in the fields of cell biology, metabolism, and molecular medicine.
Product Name:
ATP6V1E1 Rabbit Polyclonal Antibody
SKU:
CAB3756
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 77-226 of human ATP6V1E1 (NP_001687.1).
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome.
Purification Method:
Affinity purification
Gene ID:
529
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ATP6V1E1 antibody (CAB3756) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
