The ATP6V0C Monoclonal Antibody is a high-quality research tool specifically designed for studies involving the ATP6V0C protein, a subunit of the vacuolar ATPase complex. This monoclonal antibody, produced through hybridoma technology, boasts superior specificity and sensitivity for the detection of ATP6V0C in various sample types.ATP6V0C is a key component of the vacuolar ATPase complex, essential for maintaining cellular pH homeostasis and regulating vesicular trafficking. Dysregulation of ATP6V0C has been implicated in various diseases, including cancer, osteoporosis, and renal diseases.
This antibody enables precise detection and localization of ATP6V0C in experimental settings, making it a valuable tool for researchers in cell biology, cancer biology, and drug discovery.By targeting the ATP6V0C protein, this monoclonal antibody offers researchers the opportunity to delve deeper into the function and regulation of the vacuolar ATPase complex. Its reliable performance in techniques such as immunofluorescence, immunoprecipitation, and immunohistochemistry makes it an indispensable asset for elucidating the role of ATP6V0C in cellular processes and disease pathogenesis.
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. This gene encodes the V0 subunit c. Alternative splicing results in transcript variants. Pseudogenes have been identified on chromosomes 6 and 17.
Purification Method:
Affinity purification
Gene ID:
527
Clone Number:
ARC61235
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using ATP6V0C Rabbit mAb (CAB23757) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
