The ATP12A Polyclonal Antibody (CAB14372) is a powerful tool for research involving ATP12A, a protein involved in ion transport and acidification of intracellular compartments. This antibody, raised in rabbits, offers high reactivity with human samples and is validated for use in various applications, including Western blot and immunofluorescence.ATP12A plays a crucial role in maintaining cellular pH balance and regulating ion levels, making it a key player in processes such as endocytosis, phagocytosis, and lysosomal function. Dysregulation of ATP12A has been linked to diseases such as hypertension, gastric cancer, and kidney disorders, highlighting its importance in physiological and pathological conditions.
By utilizing the ATP12A Polyclonal Antibody, researchers can explore the function and regulation of ATP12A in different cell types and tissues, providing valuable insights into its role in health and disease. This antibody is a valuable tool for studies in cell biology, physiology, and cancer research, offering the ability to detect and analyze ATP12A expression with high specificity and sensitivity.
Product Name:
ATP12A Rabbit Polyclonal Antibody
SKU:
CAB14372
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 890-980 of human ATP12A (NP_001172014.1).
The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This gene encodes a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
479
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ATP12A Rabbit pAb (CAB14372) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
