The ASPM Polyclonal Antibody (PAC054138) is a valuable tool for researchers studying ASPM, a protein associated with microcephaly and brain development. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding to the ASPM protein, this antibody enables detection and analysis in a variety of cell types, making it essential for investigations in neuroscience and developmental biology.ASPM, also known as abnormal spindle-like microcephaly associated protein, plays a crucial role in regulating brain size and neural stem cell division. Mutations in the ASPM gene have been linked to microcephaly, a condition characterized by a significantly smaller head circumference and intellectual disabilities.
Research on ASPM is essential for understanding brain development and potential therapeutic interventions for neurodevelopmental disorders.By utilizing the ASPM Polyclonal Antibody, researchers can explore the functions and mechanisms of ASPM, furthering our understanding of brain development and associated disorders. This antibody is a valuable resource for studies focusing on neurodevelopment, regenerative medicine, and genetic disorders affecting brain growth.
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using PACO54138 at dilution of 1:100.
Immunofluorescent analysis of Hela cells using PACO54138 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human lung tissue using PACO54138 at dilution of 1:100.
Background:
Probable role in mitotic spindle regulation and coordination of mitotic processes. May have a preferential role in regulating neurogenesis.
Synonyms:
Abnormal spindle-like microcephaly-associated protein (Abnormal spindle protein homolog) (Asp homolog), ASPM, MCPH5
UniProt Protein Function:
ASPM: Probable role in mitotic spindle regulation and coordination of mitotic processes. May have a preferential role in regulating neurogenesis. Defects in ASPM are the cause of microcephaly primary type 5 (MCPH5); also known as true microcephaly or microcephaly vera. Microcephaly is defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. This entity is inherited as autosomal recessive trait. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Cell cycle regulationChromosomal Location of Human Ortholog: 1q31Cellular Component: microtubule; cytoplasm; midbody; nucleusMolecular Function: calmodulin bindingBiological Process: oogenesis; developmental growth; maintenance of centrosome localization; mitosis; positive regulation of neuroblast proliferation; negative regulation of neuron differentiation; negative regulation of asymmetric cell division; male gonad development; neuron migration; spermatogenesis; cerebral cortex development; forebrain neuroblast divisionDisease: Microcephaly 5, Primary, Autosomal Recessive
UniProt Protein Details:
NCBI Summary:
This gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts. Studies in mouse also suggest a role of this gene in mitotic spindle regulation, with a preferential role in regulating neurogenesis. Mutations in this gene are associated with microcephaly primary type 5. Multiple transcript variants encoding different isoforms have been found for this gene.
218, 95, 75kD Observed UniProtKB/ Swiss-Prot entry Q8IZT6 describes 2 isoforms produced by alternative splicing: Isoform 1 at 409.800 kD and Isoform 2 at 217.794 kD. Multiple other isoforms in ref: Human Molecular Genetics, 2005, Vol.14 No. 15 2155-2165.
NCBI Full Name:
ASPM protein
NCBI Synonym Full Names:
asp (abnormal spindle) homolog, microcephaly associated (Drosophila)
NCBI Official Symbol:
ASPMÂ Â
NCBI Official Synonym Symbols:
ASP; MCPH5; Calmbp1; FLJ10517; FLJ10549; FLJ43117; DKFZp686N06184Â Â
