The ASPh Polyclonal Antibody (CAB6873) is a valuable tool for researchers studying ASPh, a key enzyme involved in the regulation of protein glycosylation. This antibody, produced in rabbits, is highly specific for ASPh and is suitable for use in various research applications, including Western blotting. By targeting the ASPh protein, this antibody allows for the detection and analysis of ASPh expression in different cell types, making it an essential tool for studies in glycobiology and cancer research.
ASPh, also known as aspartyl beta-hydroxylase, plays a crucial role in the modification of glycoproteins, which are important in various cellular processes. Dysregulation of ASPh has been implicated in the development of cancer and other diseases, making it a promising target for therapeutic intervention. By understanding the function of ASPh, researchers can gain insights into the mechanisms underlying disease pathology and potentially identify new treatment strategies.
Product Name:
ASPH Rabbit Polyclonal Antibody
SKU:
CAB6873
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 81-270 of human ASPH (NP_001158227.1).
Endoplasmic reticulum membrane, Sarcoplasmic reticulum membrane, Single-pass type II membrane protein.
Calculated MW:
86kDa
Observed MW:
Refer to figures
This gene is thought to play an important role in calcium homeostasis. The gene is expressed from two promoters and undergoes extensive alternative splicing. The encoded set of proteins share varying amounts of overlap near their N-termini but have substantial variations in their C-terminal domains resulting in distinct functional properties. The longest isoforms (a and f) include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain that hydroxylates aspartic acid or asparagine residues in the epidermal growth factor (EGF)-like domains of some proteins, including protein C, coagulation factors VII, IX, and X, and the complement factors C1R and C1S. Other isoforms differ primarily in the C-terminal sequence and lack the hydroxylase domain, and some have been localized to the endoplasmic and sarcoplasmic reticulum. Some of these isoforms are found in complexes with calsequestrin, triadin, and the ryanodine receptor, and have been shown to regulate calcium release from the sarcoplasmic reticulum. Some isoforms have been implicated in metastasis.
Purification Method:
Affinity purification
Gene ID:
444
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Immunofluorescence analysis of HeLa cells using ASPH antibody (CAB6873). Blue: DAPI for nuclear staining.
