ARPC4 Antibody (PACO56318)
- SKU:
- PACO56318
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
ARPC4 Antibody (PACO56318)
The ARPC4 Polyclonal Antibody (PAC056318) is a valuable tool for researchers studying the ARPC4 protein, a key component of the actin-related protein 2/3 complex involved in cell motility and cytoskeletal rearrangement. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.ARPC4 is essential for the regulation of actin polymerization and cell migration, making it a crucial target for studies in cell biology and cancer research.
By binding to ARPC4, this antibody enables precise detection and analysis of the protein in various cell types, providing valuable insights into its function and potential roles in disease development.With its high reactivity and specificity, the ARPC4 Polyclonal Antibody is a powerful tool for researchers aiming to unravel the molecular mechanisms underlying cell movement and invasion, and its implications in pathological conditions such as cancer metastasis.
| Antibody Name: | ARPC4 Antibody (PACO56318) |
| Antibody SKU: | PACO56318 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Actin-related protein 2/3 complex subunit 4 protein (2-168AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO56318 diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO56318 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament. |
| Synonyms: | Actin-related protein 2/3 complex subunit 4 (Arp2/3 complex 20 kDa subunit) (p20-ARC), ARPC4, ARC20 |
| UniProt Protein Function: | |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes one of seven subunits of the human Arp2/3 protein complex. This complex controls actin polymerization in cells and has been conserved throughout eukaryotic evolution. This gene encodes the p20 subunit, which is necessary for actin nucleation and high-affinity binding to F-actin. Alternative splicing results in multiple transcript variants. Naturally occurring read-through transcription exists between this gene and the downstream tubulin tyrosine ligase-like family, member 3 (TTLL3), which results in the production of a fusion protein. [provided by RefSeq, Nov 2010] |
| UniProt Code: | P59998 |
| NCBI GenInfo Identifier: | 311771642 |
| NCBI Gene ID: | 10093 |
| NCBI Accession: | NM_005718.4 |
| UniProt Secondary Accession: | P59998 |
| UniProt Related Accession: | P59998 |
| Molecular Weight: | |
| NCBI Full Name: | Homo sapiens actin related protein 2/3 complex subunit 4 (ARPC4), transcript variant 1, mRNA |
| NCBI Synonym Full Names: | actin related protein 2/3 complex subunit 4 |
| NCBI Official Symbol: | ARPC4Â Â |
| NCBI Official Synonym Symbols: | ARC20; P20-ARCÂ Â |
| NCBI Protein Information: | actin-related protein 2/3 complex subunit 4 |
| UniProt Protein Name: | Actin-related protein 2/3 complex subunit 4 |
| UniProt Synonym Protein Names: | Arp2/3 complex 20 kDa subunit |
| Protein Family: | Actin-related protein 2/3 complex |
| UniProt Gene Name: | ARPC4Â Â |
| UniProt Entry Name: | ARPC4_HUMAN |
| Antibodies |
| ARPC4 Antibody (PACO07862) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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