The ARIH2 Polyclonal Antibody (PAC019136) is a valuable tool for researchers studying the ARIH2 protein, an E3 ubiquitin ligase involved in the ubiquitination and degradation of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity for human samples, making it ideal for Western blot applications. By specifically binding to ARIH2, this antibody enables researchers to detect and analyze the protein in various cell types, providing insights into its role in processes such as protein degradation, cell cycle regulation, and DNA damage response.ARIH2, also known as TRIAD1, is a key player in the ubiquitin-proteasome system, which regulates the turnover of proteins and maintains cellular homeostasis.
Dysregulation of ARIH2 has been implicated in various diseases, including cancer, neurodegenerative disorders, and immune-related conditions. Therefore, further understanding of ARIH2 function and regulation is crucial for developing targeted therapies and gaining insights into disease mechanisms.Overall, the ARIH2 Polyclonal Antibody (PAC019136) is a valuable asset for researchers investigating the role of ARIH2 in cellular processes and disease pathogenesis. Its specificity, sensitivity, and versatility make it a reliable tool for elucidating the functions of ARIH2 and exploring its potential as a therapeutic target.
Antibody Name:
ARIH2 Antibody (PACO19136)
Antibody SKU:
PACO19136
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:5000, WB:1:500-1:2000
Species Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide of human ARIH2
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-5: Hela cells, Jurkat cells, 293T cells, K562 cells, mouse testis tissue, Primary antibody: PACO19136(ARIH2 Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds.
Background:
TRIAD1, also known as ARIH2 (ariadne homolog 2) or ARI2, is a 493 amino acid, protein that contains one IBR-type zinc finger and two RING-type zinc fingers and belongs to the ariadne subfamily of RBR proteins. Localized to the nucleus, TRIAD1 interacts with UBE2L3 and is thought to act as an E3 ubiquitin-protein ligase, functioning to accept ubiquitin from E2 ubiquitin-conjugating enzymes and transfer the acquired ubiquitin residue to target substrates. TRIAD1 is subject to post-translational DNA damage-dependent phosphorylation, probably by ATM or ATR. The gene encoding TRIAD1 maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci.
Synonyms:
ariadne homolog 2 (Drosophila)
UniProt Protein Function:
ARIH2: E3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys- 63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis. Belongs to the RBR family. Ariadne subfamily.Protein type: Ubiquitin conjugating system; Ubiquitin ligase; Ligase; EC 6.3.2.-Chromosomal Location of Human Ortholog: 3p21Cellular Component: cytoplasm; nucleus; ubiquitin ligase complexMolecular Function: protein binding; ubiquitin conjugating enzyme binding; ubiquitin-protein ligase activityBiological Process: developmental cell growth; multicellular organismal development; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is an E3 ubiquitin-protein ligase that polyubiquitinates some proteins, tagging them for degradation. The encoded protein upregulates p53 in some cancer cells and may inhibit myelopoiesis. Several transcript variants encoding different isoforms have been found for this gene, although the full-length nature of some of them have not been determined yet. [provided by RefSeq, Nov 2015]
