The ARHGEF10 Polyclonal Antibody (PAC015513) is a valuable tool for researchers studying ARHGEF10, a protein known for its role in cell signaling pathways and cytoskeletal organization. This antibody, generated in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blotting applications. By specifically binding to the ARHGEF10 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it well-suited for investigations in molecular biology and cancer research.ARHGEF10, also known as Rho guanine nucleotide exchange factor 10, plays a crucial role in regulating the activity of Rho GTPases, which are key regulators of cell morphology and migration.
Dysregulation of ARHGEF10 has been linked to various diseases, including cancer, neurodevelopmental disorders, and cardiovascular conditions. Therefore, studying the function of ARHGEF10 is essential for gaining insights into these pathologies and developing targeted therapies.By utilizing the ARHGEF10 Polyclonal Antibody, researchers can deepen their understanding of the molecular mechanisms involving ARHGEF10 and its impact on cellular processes. This antibody serves as a valuable tool for investigating the role of ARHGEF10 in health and disease, ultimately contributing to advancements in biomedicine and therapeutic interventions.
Antibody Name:
ARHGEF10 Antibody (PACO15513)
Antibody SKU:
PACO15513
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:50-1:200
Species Reactivity:
Human, Mouse
Immunogen:
Fusion protein of human ARHGEF10
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using PACO15513(ARHGEF10 Antibody) at dilution 1/45, on the right is treated with fusion protein. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using PACO15513(ARHGEF10 Antibody) at dilution 1/45, on the right is treated with fusion protein. (Original magnification: x200).
Background:
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. ARHGEF10 (rho guanine nucleotide exchange factor 10), also known as GEF10 or KIAA0294, is a 1,369 amino acid, protein that contains one DBL-homology domain and is thought to play a role in myelination of peripheral nerves, specifically during development. ARHGEF10 is expressed as four alternatively spliced isoforms that are present at low levels in ovary, lung, testis and kidney, with considerably higher expression in the central and peripheral nervous systems.
Synonyms:
Rho guanine nucleotide exchange factor (GEF) 10
UniProt Protein Function:
ARHGEF10: May play a role in developmental myelination of peripheral nerves. Defects in ARHGEF10 are the cause of slowed nerve conduction velocity (SNCV). Affected individuals present a reduction in nerve conduction velocities without any clinical signs of peripheral or central nervous system dysfunction. SNCV inheritance is autosomal dominant. 5 isoforms of the human protein are produced by alternative splicing.Protein type: GEFs, Rac/Rho; GEFsChromosomal Location of Human Ortholog: 8p23Cellular Component: centrosome; cytosolMolecular Function: Rho guanyl-nucleotide exchange factor activity; protein binding; kinesin bindingBiological Process: positive regulation of stress fiber formation; centrosome duplication; myelination in the peripheral nervous systemDisease: Slowed Nerve Conduction Velocity, Autosomal Dominant
UniProt Protein Details:
NCBI Summary:
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. [provided by RefSeq, Jul 2008]
