The ARHGDIG Polyclonal Antibody (PACO47014) is a valuable tool for researchers studying ARHGDIG, a protein involved in the regulation of cell migration and invasion. This antibody, produced in rabbits, is highly specific for ARHGDIG in human samples and has been validated for use in Western blotting applications. By binding to the ARHGDIG protein, this antibody allows for accurate detection and analysis in various cell types, making it an essential component for studies in cell biology, cancer research, and drug development.ARHGDIG, also known as Rho GDP-dissociation inhibitor 3 (RhoGDI-3), is a key player in the control of Rho GTPases, which are critical regulators of cell migration and invasion.
Dysregulation of ARHGDIG has been linked to various diseases, including cancer and neurodegenerative disorders. By understanding the role of ARHGDIG in these conditions, researchers can develop new therapeutic strategies targeting cell motility and invasion, ultimately leading to the development of novel treatments for these diseases. Overall, the ARHGDIG Polyclonal Antibody (PACO47014) is a valuable tool for researchers looking to gain insights into the functions of ARHGDIG and its implications in disease pathology, providing a stepping stone towards potential therapeutic interventions.
Western blot. All lanes: ARHGDIG antibody at 2 µg/ml. Lane 1: Mouse brain tissue. Lane 2: Mouse lung tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 25 kDa. Observed band size: 25, 50 kDa..
Immunohistochemistry of paraffin-embedded human placenta tissue using PACO47014 at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human brain tissue using PACO47014 at dilution of 1:100.
Background:
Inhibits GDP/GTP exchange reaction of RhoB. Interacts specifically with the GDP- and GTP-bound forms of post-translationally processed Rhob and Rhog proteins, both of which show a growth-regulated expression in mammalian cells. Stimulates the release of the GDP-bound but not the GTP-bound RhoB protein. Also inhibits the GDP/GTP exchange of RhoB but shows less ability to inhibit the dissociation of prebound GTP.
RhoGDI gamma: Inhibits GDP/GTP exchange reaction of RhoB. Interacts specifically with the GDP- and GTP-bound forms of post- translationally processed Rhob and Rhog proteins, both of which show a growth-regulated expression in mammalian cells. Stimulates the release of the GDP-bound but not the GTP-bound RhoB protein. Also inhibits the GDP/GTP exchange of RhoB but shows less ability to inhibit the dissociation of prebound GTP. Belongs to the Rho GDI family.Protein type: Inhibitor; Motility/polarity/chemotaxis; G protein regulator, misc.; Cell adhesionChromosomal Location of Human Ortholog: 16p13.3Cellular Component: membrane; cytoplasmic membrane-bound vesicle; cytosolMolecular Function: Rho GDP-dissociation inhibitor activity; GTPase activator activityBiological Process: regulation of small GTPase mediated signal transduction; regulation of protein localization; small GTPase mediated signal transduction; negative regulation of cell adhesion; Rho protein signal transduction; positive regulation of GTPase activity
UniProt Protein Details:
NCBI Summary:
The GDP-dissociation inhibitors (GDIs) play a primary role in modulating the activation of GTPases by inhibiting the exchange of GDP for GTP. See ARHGDIB (MIM 602843).[supplied by OMIM, Nov 2010]
