The ARHGAP32 Polyclonal Antibody (PAC061622) is an essential tool for researchers studying ARHGAP32, a protein involved in cell signaling and cytoskeleton organization. This antibody, produced in rabbits, shows high specificity and sensitivity towards human samples, making it suitable for Western blot applications. By binding to the ARHGAP32 protein, this antibody facilitates the detection and analysis of ARHGAP32 in various cell types, offering valuable insights for studies in cell biology and cancer research.ARHGAP32, also known as Rho GTPase-activating protein 32, plays a crucial role in regulating Rho GTPases, which are key regulators of cellular functions such as cell morphology, motility, and division.
Dysregulation of ARHGAP32 has been linked to various diseases, including cancer, neurodevelopmental disorders, and cardiovascular diseases. Therefore, unraveling the functions of ARHGAP32 is essential for understanding the underlying mechanisms of these conditions and developing targeted therapies.In summary, the ARHGAP32 Polyclonal Antibody (PAC061622) serves as a reliable tool for researchers aiming to explore the role of ARHGAP32 in cellular processes and disease pathogenesis. Its high specificity and sensitivity, along with its versatility in different applications, make it a valuable asset for advancing scientific knowledge in the fields of cell biology and medical research.
Antibody Name:
ARHGAP32 Antibody (PACO61622)
Antibody SKU:
PACO61622
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:20-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Rho GTPase-activating protein 32 protein (130-205AA)
Immunofluorescence staining of SH-SY5Y cells with PACO61622 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
GTPase-activating protein (GAP) promoting GTP hydrolysis on RHOA, CDC42 and RAC1 small GTPases. May be involved in the differentiation of neuronal cells during the formation of neurite extensions. Involved in NMDA receptor activity-dependent actin reorganization in dendritic spines. May mediate cross-talks between Ras- and Rho-regulated signaling pathways in cell growth regulation. Isoform 2 has higher GAP activity (By similarity).
Synonyms:
Rho GTPase-activating protein 32, Brain-specific Rho GTPase-activating protein, GAB-associated Cdc42/Rac GTPase-activating protein, GC-GAP, GTPase regulator interacting with TrkA, Rho-type GTPase-activating protein 32, Rho/Cdc42/Rac GTPase-activating protein RICS, RhoGAP involved in the beta-catenin-N-cadherin and NMDA receptor signaling, p200RhoGAP, p250GAP, ARHGAP32, GRIT, KIAA0712, RICS
UniProt Protein Function:
ARHGAP32: GTPase-activating protein (GAP) promoting GTP hydrolysis on RHOA, CDC42 and RAC1 small GTPases. May be involved in the differentiation of neuronal cells during the formation of neurite extensions. Involved in NMDA receptor activity-dependent actin reorganization in dendritic spines. May mediate cross-talks between Ras- and Rho-regulated signaling pathways in cell growth regulation. Isoform 2 has higher GAP activity. Belongs to the PX domain-containing GAP family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: GAPs; GAPs, Rac/RhoChromosomal Location of Human Ortholog: 11q24.3Cellular Component: Golgi membrane; postsynaptic membrane; endoplasmic reticulum membrane; postsynaptic density; dendritic spine; endosome membrane; cell cortex; cytosol; cell junction; actin cytoskeletonMolecular Function: protein binding; GTPase activator activity; phosphoinositide bindingBiological Process: regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of GTPase activity
UniProt Protein Details:
NCBI Summary:
RICS is a neuron-associated GTPase-activating protein that may regulate dendritic spine morphology and strength by modulating Rho GTPase (see RHOA; MIM 165390) activity (Okabe et al., 2003 [PubMed 12531901]).[supplied by OMIM, Mar 2008]
